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What are the steps of IVF Process?

The process of becoming pregnant through in vitro fertilization IVF takes a few steps. Understanding as much IVF information as you can will help you prepare. There are four basic IVF steps in the IVF process in Pune.


Before the test tube baby process in Pune can begin, you will need to be evaluated. This will involve testing and ovarian stimulation to make sure there are no other issues that are going to require surgical repair. The pre-cycle tests include a hormonal evaluation to assess thyroid function and ovarian reserve. A screening of both partners will take place which will test for sexually transmitted infections, and the male will undergo a semen analysis. Most women will take fertility medications to stimulate the ovaries for 8 to 14 days. The ovary stimulation is done to get the eggs mature and ready to retrieve. Normally, about 10 to 20 eggs are retrieved, but typically only about two-thirds of them are going to be viable. The stimulation process will be carefully monitored by your physician to prevent ovarian hyper-stimulation syndrome OHSS. Your monitoring will involve hormonal tests and vaginal ultrasounds.

Retrieving the Egg

When the time is ready, usually between 34 to 36 hours after getting the trigger shot before ovulation, you will undergo a surgical procedure to retrieve the eggs. This is done by using a very thin needle which is guided by use of ultrasound through the top of the vagina and into one ovary at a time. The follicular fluid is removed using suction to bring the eggs out with the fluid. To alleviate discomfort or pain, you will be given an anaesthesia through an IV. The entire process takes about half an hour.


Now that the eggs have been retrieved and placed in an incubator, it is time to bring together the viable egg, or eggs, with the sperm. Basically, there are two methods to fertilize the egg. The first one is through conventional insemination. The other option is intra-cytoplasmic injection ICSI. The method you use will be determined by your IVF team based on your specific situation. The conventional insemination places the sperm in a culture medium inside a petri dish with the egg. Both the sperm and the egg is then incubated together in the lab allowing the sperm to enter the egg on its own. The ICSI method, on the other hand, uses one sperm which is injected into the egg. Regardless of which method is used, the egg will be checked the following day to see if it has become fertilized.

Transfer of the Embryo

If everything has been a success to this point, it is time for the final stage before pregnancy can take place. It’s time to transfer the embryo into the uterus. This will happen usually within a month or two after retrieval. In the meantime, anywhere between one to six days, typically 3 to 5 days after the egg is retrieved, your IVF team will determine when the embryo will be able to be transferred. If genetic testing is being done, this will occur on day 5 or 6 and the embryo is frozen until the test results come back. At this point, the embryo is thawed and transferred into the woman’s uterus using a flexible, thin catheter. During the procedure, an abdominal ultrasound will be used to make sure the embryo is properly placed. This step is usually no more painful than a pap smear. Occasionally, something called assisted hatching is done to help make the embryo more likely to implant.

Finally, after about 12 days, you will return to the office to have your blood tested for pregnancy. If everything was successful, you will then be referred to an obstetrician for prenatal care to prepare for the birth of your baby.